Tirzepatide Reconstitution
How to mix Tirzepatide and read the exact units to draw. Enter your vial size and bacteriostatic water below for the concentration and dose on a U-100 insulin syringe.
How to reconstitute Tirzepatide
- Swab the rubber stopper of the Tirzepatide vial and the bacteriostatic water vial with an alcohol wipe.
- Draw 1.5 ml of bacteriostatic water into a syringe.
- Inject it slowly down the inside wall of the Tirzepatide vial, not directly onto the powder.
- Swirl gently until the powder fully dissolves. Do not shake. Shaking can damage the peptide.
- No manufacturer stability data exists for reconstituted lyophilised tirzepatide.
Tirzepatide concentration by water volume
Tirzepatide is commonly supplied in 30 mg vials. The concentration depends only on how much bacteriostatic water you add. More water lowers the concentration and makes small doses easier to measure.
| BAC water added | Concentration | Per 1 unit |
|---|---|---|
| 1 ml | 30,000 mcg/ml | 300 mcg |
| 1.5 ml | 20,000 mcg/ml | 200 mcg |
| 3 ml | 10,000 mcg/ml | 100 mcg |
Tirzepatide units to draw
At 20,000 mcg/ml (a 30 mg vial in 1.5 ml of bacteriostatic water), here is the draw for doses across the range Tirzepatide is typically used at. Change any number in the calculator above for your own vial.
| Dose | Units to draw | Volume |
|---|---|---|
| 2.5 mg | 12.5 units | 0.12 ml |
| 5 mg | 25 units | 0.25 ml |
| 7.5 mg | 37.5 units | 0.38 ml |
| 10 mg | 50 units | 0.50 ml |
| 15 mg | 75 units | 0.75 ml |
Tirzepatide dosing
Tirzepatide starts at 2.5 mg once weekly, a tolerability dose rather than a therapeutic one, and steps up by 2.5 mg no sooner than every four weeks toward a maximum of 15 mg, an escalation that spans roughly 20 weeks. As with semaglutide, the slow ramp exists to keep the gastrointestinal side effects manageable, and pushing the dose faster does not improve the outcome. Enter your own vial size, water volume, and target dose in the calculator above to convert any Tirzepatide protocol into exact insulin-syringe units. For the full profile, see the Tirzepatide compound page.
Storing reconstituted Tirzepatide
No manufacturer stability data exists for reconstituted lyophilised tirzepatide. Refrigerate at 2 to 8 degrees C, do not freeze, and protect from light. The label figures that do exist (21 days at up to 30 degrees C for single-dose pens, 30 days for the KwikPen after first use) describe a ready-to-use solution, not a vial you mixed yourself.
Tirzepatide is dosed in milligrams, not micrograms. Approved product is supplied as ready-to-use single-dose pens and vials, so the vial size and water volume here are research-supply convention. Label titration starts at 2.5 mg weekly for 4 weeks, which is an initiation dose and not a maintenance dose, then rises in 2.5 mg steps at intervals of at least 4 weeks to a maximum of 15 mg.
The dose range above comes from approved prescribing information for Tirzepatide.
The math
- Concentration (mcg/ml) = vial size in mg × 1,000 ÷ bacteriostatic water in ml.
- Volume for your dose (ml) = dose in mcg ÷ concentration.
- Units to draw = volume in ml × 100 on a U-100 insulin syringe.
Prefer a full workspace? The peptide dosage & reconstitution calculator adds blend, converter, half-life, and titration tools.
Tirzepatide reconstitution FAQ
Draw 1.5 ml of bacteriostatic water into a syringe, inject it slowly down the inside wall of the Tirzepatide vial, and swirl gently until the powder dissolves. Do not shake. The concentration is the vial size in mg times 1,000, divided by the millilitres you added.
1.5 ml for a 30 mg vial gives 20,000 mcg/ml. More liquid makes small doses easier to measure accurately. The calculator shows the concentration for any volume you choose.
Units to draw equals your dose in mcg divided by the concentration in mcg/ml, times 100 on a U-100 insulin syringe. At 20,000 mcg/ml, a 7.5 mg dose is 37.5 units.
No manufacturer stability data exists for reconstituted lyophilised tirzepatide. Refrigerate at 2 to 8 degrees C, do not freeze, and protect from light. The label figures that do exist (21 days at up to 30 degrees C for single-dose pens, 30 days for the KwikPen after first use) describe a ready-to-use solution, not a vial you mixed yourself.
References
Track Tirzepatide in Peptide AI
Log your doses, connect labs and wearables, and let the AI show you how Tirzepatide is affecting your biomarkers over time.
Download the appEducational information only. This is not medical advice. Peptide AI is an informational and tracking platform. Everything on this page is for general education. It is not a substitute for professional medical advice and is not a recommendation to use any compound. Many peptides listed here are research compounds the FDA has not approved for human use. Talk to a qualified, licensed healthcare provider before you start, change, or stop any protocol.