Retatrutide Reconstitution

How to reconstitute Retatrutide

1. Swab the rubber stopper of the Retatrutide vial and the bacteriostatic water vial with an alcohol wipe.
2. Draw 1 ml of bacteriostatic water into a syringe.
3. Inject it slowly down the inside wall of the Retatrutide vial, not directly onto the powder.
4. Swirl gently until the powder fully dissolves. Do not shake. Shaking can damage the peptide.
5. No stability data has been published for reconstituted retatrutide.

Retatrutide concentration by water volume

Retatrutide is commonly supplied in 20 mg vials. The concentration depends only on how much bacteriostatic water you add. More water lowers the concentration and makes small doses easier to measure.

Retatrutide units to draw

At 20,000 mcg/ml (a 20 mg vial in 1 ml of bacteriostatic water), here is the draw for doses across the range Retatrutide is typically used at. Change any number in the calculator above for your own vial.

Retatrutide dosing

Because retatrutide is not approved, there is no official prescribing schedule. Trials used once-weekly subcutaneous injection with gradual dose escalation, testing 1, 4, 8, and 12 mg arms. The higher doses drove the biggest weight loss but also the most gastrointestinal side effects, following the same titration logic as the approved incretin drugs. Enter your own vial size, water volume, and target dose in the calculator above to convert any Retatrutide protocol into exact insulin-syringe units. For the full profile, see the Retatrutide compound page.

Storing reconstituted Retatrutide

No stability data has been published for reconstituted retatrutide. Refrigerate at 2 to 8 degrees C and protect from light. How long it remains stable is unknown, and no day count is given here because none has been established.

Not approved by any regulator. The doses above are the randomised arms of the phase 2 obesity trial (Jastreboff et al., NEJM 2023): 1, 4, 8, and 12 mg once weekly, with 2 mg and 4 mg used during escalation. Vial size and water volume are research-supply convention with no authoritative source.

The dose range above comes from published clinical trials. Retatrutide is not FDA-approved for this use.

The math

• Concentration (mcg/ml) = vial size in mg × 1,000 ÷ bacteriostatic water in ml.
• Volume for your dose (ml) = dose in mcg ÷ concentration.
• Units to draw = volume in ml × 100 on a U-100 insulin syringe.

Prefer a full workspace? The peptide dosage & reconstitution calculator adds blend, converter, half-life, and titration tools.

Retatrutide reconstitution FAQ

References

• https://www.nejm.org/doi/full/10.1056/NEJMoa2301972
• https://clinicaltrials.gov/study/NCT04881760