BPC-157 Reconstitution
How to mix BPC-157 and read the exact units to draw. Enter your vial size and bacteriostatic water below for the concentration and dose on a U-100 insulin syringe.
How to reconstitute BPC-157
- Swab the rubber stopper of the BPC-157 vial and the bacteriostatic water vial with an alcohol wipe.
- Draw 2 ml of bacteriostatic water into a syringe.
- Inject it slowly down the inside wall of the BPC-157 vial, not directly onto the powder.
- Swirl gently until the powder fully dissolves. Do not shake. Shaking can damage the peptide.
- Keep the lyophilised powder at 2 to 8 degrees C, where it is stable for roughly 24 months.
BPC-157 concentration by water volume
BPC-157 is commonly supplied in 5 mg vials. The concentration depends only on how much bacteriostatic water you add. More water lowers the concentration and makes small doses easier to measure.
| BAC water added | Concentration | Per 1 unit |
|---|---|---|
| 1 ml | 5,000 mcg/ml | 50 mcg |
| 2 ml | 2,500 mcg/ml | 25 mcg |
| 3 ml | 1,666.7 mcg/ml | 16.7 mcg |
BPC-157 units to draw
At 2,500 mcg/ml (a 5 mg vial in 2 ml of bacteriostatic water), here is the draw for doses across the range BPC-157 is typically used at. Change any number in the calculator above for your own vial.
| Dose | Units to draw | Volume |
|---|---|---|
| 200 mcg | 8 units | 0.08 ml |
| 250 mcg | 10 units | 0.10 ml |
| 300 mcg | 12 units | 0.12 ml |
| 400 mcg | 16 units | 0.16 ml |
| 500 mcg | 20 units | 0.20 ml |
BPC-157 dosing
Because there are no approved human indications, there is no established clinical dose. Preclinical work and community protocols commonly reference roughly 200 to 500 mcg per day by subcutaneous injection, given either near the injury or systemically. Those numbers come from animal-to-human extrapolation and anecdote, not from dose-finding trials, which is a meaningful distinction. Enter your own vial size, water volume, and target dose in the calculator above to convert any BPC-157 protocol into exact insulin-syringe units. For the full profile, see the BPC-157 compound page.
Storing reconstituted BPC-157
Keep the lyophilised powder at 2 to 8 degrees C, where it is stable for roughly 24 months. Once mixed with bacteriostatic water, refrigerate at 2 to 8 degrees C and use within about 28 to 30 days. That window comes from the 0.9% benzyl alcohol preservative in the water, not from any published BPC-157 stability study. Protect from light and do not freeze the reconstituted solution.
There is no FDA-approved product and no published human injectable dose. In September 2023 the FDA placed BPC-157 in Category 2 of the interim 503A bulks list, meaning it may not be used in compounding because the agency identified significant safety risks; a PCAC review is scheduled for July 2026. The only human work used oral and enema formulations in ulcerative colitis trials whose dose-finding data were never published. The range above is community protocol, not a clinical dose.
There is no approved human dose for BPC-157. The range above reflects preclinical work and community protocols, not dose-finding trials, which is a meaningful distinction.
The math
- Concentration (mcg/ml) = vial size in mg × 1,000 ÷ bacteriostatic water in ml.
- Volume for your dose (ml) = dose in mcg ÷ concentration.
- Units to draw = volume in ml × 100 on a U-100 insulin syringe.
Prefer a full workspace? The peptide dosage & reconstitution calculator adds blend, converter, half-life, and titration tools.
BPC-157 reconstitution FAQ
Draw 2 ml of bacteriostatic water into a syringe, inject it slowly down the inside wall of the BPC-157 vial, and swirl gently until the powder dissolves. Do not shake. The concentration is the vial size in mg times 1,000, divided by the millilitres you added.
2 ml for a 5 mg vial gives 2,500 mcg/ml. More liquid makes small doses easier to measure accurately. The calculator shows the concentration for any volume you choose.
Units to draw equals your dose in mcg divided by the concentration in mcg/ml, times 100 on a U-100 insulin syringe. At 2,500 mcg/ml, a 300 mcg dose is 12 units.
Keep the lyophilised powder at 2 to 8 degrees C, where it is stable for roughly 24 months. Once mixed with bacteriostatic water, refrigerate at 2 to 8 degrees C and use within about 28 to 30 days. That window comes from the 0.9% benzyl alcohol preservative in the water, not from any published BPC-157 stability study. Protect from light and do not freeze the reconstituted solution.
References
Track BPC-157 in Peptide AI
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Download the appEducational information only. This is not medical advice. Peptide AI is an informational and tracking platform. Everything on this page is for general education. It is not a substitute for professional medical advice and is not a recommendation to use any compound. Many peptides listed here are research compounds the FDA has not approved for human use. Talk to a qualified, licensed healthcare provider before you start, change, or stop any protocol.